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KMID : 0903619910320020137
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Journal of the Korean Society for Horticultural Science
1991 Volume.32 No. 2 p.137 ~ p.145
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Micropropagation of Rootstock and Production of Interspecific Hybirds by Embryo Culture in Cucurbita
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조영환/Cho, Young Hwan
백기엽/황주광/서영기/Paek, Kee Yoeup/Hwang, Ju Kwang/Seo, Young Kee
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Abstract
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Experiments were conducted to develop a micropropagation system for rootstock plants, and breeding procedures for obtaining interspecific hybridization by embryo culture in Cucurbita. Some of the characteristics of interspecific hybrids obtained were also investigated. Embryo germination in Cucurbita ficifolia for stock plant was favorable on the Murashige-Skoog media containing 6.0% surose, and successful multiple shoots could be obtained by reculturing shoot tips taken which were from germinated seedlings in the MS media supplemented with 0.5 to 1.0㎎/ℓ BAP. No differentiation of shoots and roots occurred in hypocotyl segment culture. Transplantable plantlets to soil were obtained when shoot tips were cultured on the MS media containing 1.0㎎/ℓ BAP and 0.5㎎/ℓ IBA or 0.5∼3.0㎎/ℓ IAA in combinations. Percent of fruit set and embryo size were greatly influenced by pollen parent and/or variety. In interspecific cross of C. maxima `Myako´ × C. moschata `Butter Bush,´ C. moschata `PM 142´ × C. maxima `Myako,´ C. moschata × Shindosa and Shindosa × C. moschata `PM 142´ , it was desirable to employ an immature embryo culture technique for obtaining interspecific hybrids due to the very low frequency of normal embryo development in vivo. Male (C. maximax×C. moschata) and female (Shindosa × C. mixta) flowering in vitro could be observed. Interspecific hybrid obtained by crossing C. maxima × C. moschata had a high incidence of female flowers. Dark green color and conical shape in fruits by most of interspecific hybrids were assumed to be dominant to a yellow color and flatness.
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